Journal: Cellular & Molecular Biology Letters

Article Title: PCSK9 inhibition ameliorates microplastic-induced endothelial redox imbalance via SIRT6 modulation

doi: 10.1186/s11658-025-00838-z

Figure Lengend Snippet: Effects of MPs on teloHAEC. Cell viability evaluated in teloHAEC exposed to different concentrations of A PE and B PVC (0–70 µg/mL) for 24, 48, and 72 h. C , D Cell viability assessed after 48 h of treatment with PE or PVC (30 µg/mL) and increasing concentration of PVC or PE (0–70 µg/mL), respectively. Evaluation of E MCP-1, F VCAM1, and G ICAM1 content in teloHAEC treated with PE (70 µg/mL), PVC (70 µg/mL), or PE + PVC (30 µg/mL + 30 µg/mL) for 48 h. H , I Representative cell cycle detection by FACS analysis in EC exposed to MPs. Data expressed as mean ± standard deviation (SD) of n = 4 independent experiments. * p < 0.05 versus 0 µg/mL or Ctr; ** p < 0.01 versus 0 µg/mL or Ctr; • p < 0.001 versus 0 µg/mL or Ctr; † p < 0.05 versus PE; ‡ p < 0.05 versus PVC; ns, not significant versus PE and PVC

Article Snippet: The levels of inflammatory mediators MCP-1 (RAF081R, BioVendor, Brno, Czech Republic), VCAM1 (EH0326, FineTest, Hubei, China), and ICAM1 (EH0161, FineTest, Hubei, China) were determined in cell culture supernatant, as previously reported [ ].

Techniques: Concentration Assay, Standard Deviation

Journal: Cellular & Molecular Biology Letters

Article Title: PCSK9 inhibition ameliorates microplastic-induced endothelial redox imbalance via SIRT6 modulation

doi: 10.1186/s11658-025-00838-z

Figure Lengend Snippet: iPCSK9 opposed the MP-related inflammation. A TeloHAEC viability evaluated after treatment with PE and PVC alone or combined PE + PVC, or pretreated with iPCSK9 (100 ng/mL) for 8 h and then exposed to PE, PVC, and PE + PVC. B Immunoblotting analysis of SIRT6 protein levels and ELISA assays of C MCP-1, D VCAM1, and E ICAM1. F Representative annexin V-FITC and PI-staining detected by FACS analysis and G cell cycle investigation. Data expressed as mean ± SD of n = 4 experiments. M, molecular weight markers; lane 1, Ctr; lane 2, PE; lane 3, PVC; lane 4, PE + PVC; lane 5, iPCSK9; lane 6, PE + iPCSK9; lane 7, PVC + iPCSK9; lane 8, PE + PVC + iPCSK9. * p < 0.05 versus Ctr; ** p < 0.01 versus Ctr; • p < 0.001 versus Ctr; † p < 0.05 versus PE; ‡ p < 0.05 versus PVC; + p < 0.05 versus PE + PVC; ¶ p < 0.01 versus PE; § p < 0.01 versus PVC; ▲ p < 0.01 versus PE + PVC; ns, not significant versus PE and PVC

Article Snippet: The levels of inflammatory mediators MCP-1 (RAF081R, BioVendor, Brno, Czech Republic), VCAM1 (EH0326, FineTest, Hubei, China), and ICAM1 (EH0161, FineTest, Hubei, China) were determined in cell culture supernatant, as previously reported [ ].

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Staining, Molecular Weight

Journal: Cellular & Molecular Biology Letters

Article Title: PCSK9 inhibition ameliorates microplastic-induced endothelial redox imbalance via SIRT6 modulation

doi: 10.1186/s11658-025-00838-z

Figure Lengend Snippet: SIRT6 silencing opposed the effects of iPCSK9 on inflammation and apoptosis. A Immunoblotting analysis of SIRT6 protein levels in teloHAEC treated with the empty transfection reagent (Vehicle) or transfected with negative control siRNA (NT) or with SIRT6 siRNA (siSIRT6). M, molecular weight markers; lane 1, Ctr; lane 2, Vehicle; lane 3, NT; lane 4, siSIRT6. B Immunoblotting analysis of PCSK9 protein levels and ELISA assays of C MCP-1, D VCAM1 and E ICAM1. F , G Representative dot plots and FACS analysis of annexin V-FITC and PI-staining of EC transfected with NT or siSIRT6 and exposed to PE + PVC alone or pretreated with iPCSK9 (100 µg/mL) before MP stimulation. M, molecular weight markers; lane 1, Ctr; lane 2, NT; lane 3, NT + PE + PVC; lane 4, NT + iPCSK9; lane 5, NT + PE + PVC + iPCSK9; lane 6, siSIRT6; lane 7, siSIRT6 + PE + PVC; lane 8, siSIRT6 + iPCSK9; lane 9, siSIRT6 + PE + PVC + iPCSK9. Q1: necrotic cells; Q2: late apoptotic cells; Q3: early apoptotic cells; Q4: viable cells. Data are expressed as mean ± SD of n = 3 experiments. ° p < 0.01 versus NT; # p < 0.001 versus NT; + p < 0.05 versus PE + PVC; ▲ p < 0.01 versus PE + PVC

Article Snippet: The levels of inflammatory mediators MCP-1 (RAF081R, BioVendor, Brno, Czech Republic), VCAM1 (EH0326, FineTest, Hubei, China), and ICAM1 (EH0161, FineTest, Hubei, China) were determined in cell culture supernatant, as previously reported [ ].

Techniques: Western Blot, Transfection, Negative Control, Molecular Weight, Enzyme-linked Immunosorbent Assay, Staining